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The second reaction cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to produce ferric enzyme, NO, citrulline, and water as products (Fig. 9) - UV/Vis. As drawn in Fig - https://www.livebinders.com/b/3570027?tabid=514355ed-03f4-acee-f8e7-d79f6b7bffab. 9, the response may also involve electron transfer from H4B to the Fe, IIO2 intermediate
l9 As displayed in Fig. 9, this could be since the H4B radical acts as an electron sink in a later point in the response, so regarding permit NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.